Spatial structure of tetrapeptide N-AC-Ser-Phe-Val-Gly-OMe in "protein-micelle of sodium dodecyl sulfate" complex and in solid state by NMR spectroscopy
Abstract
In the present paper the applicability of a structure determination for a four amino acid residues containing oligopeptide N-Ac-Ser-Phe-Val-Gly-OMe was investigated. The spatial structure of the "tetrapeptide (N-Ac-Ser-Phe-Val-Gly-OMe) - sodium dodecyl sulfate micelle" complex in aqueous solution was studied by 2D nuclear magnetic resonance (NMR) spectroscopy. The complexation was confirmed NOEs signs and values in the presence of sodium dodecyl sulfate. The spatial structure of the tetrapeptide in the complex was determined by 2D NOESY NMR spectroscopy. In present paper by the comparison 13C NMR chemical shifts was shown that tetra peptide's spatial structure in solid state and tetrapeptide structure in "peptide-micelle" complex are identical.
Supporting agencies: This work was supported by the Ministry of Education and Science of the Russian Federation (state target of KFU, part 2, code 2.2792.2011) and by RFBRand the Tatarstan Academy of Sciences (project No 13-03-97041).
About the Authors
D. S. Blokhin
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya, 18, Kazan 420008
S. Berger
University of Leipzig
Germany
Germany
Augustusplatz 10, 04109 Leipzig
V. V. Klochkov
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya, 18, Kazan 420008
References
1. Boman H.G. Annu. Rev. Immunol. 13, 61 (1995)
2. Zasloff M. Nature 415, 389 (2002)
3. Wang Z., Wang G. Nucleic Acids Res. 32, 590 (2004)
4. Epand R.M., Vogel H.J. Biochim. Biophys. Acta. 1462, 11 (1999)
5. Ernst R.R., Bodenhausen B., Wokaun A. Principles of Nuclear Magnetic Resonance in One and Two Dimensions, Oxford University Press, Oxford (1987)
6. Keeler J. Understanding NMR spectroscopy, Wiley Chichester (2005)
7. Blokhin D.S., Efimov S.V., Klochkov A.V., Yulmetov A.R., Filippov A.V., Antzutkin O.N., Aganov A.V., Klochkov V.V. Appl. Magn. Reson. 41(2), 267 (2011)
8. Bloсhin D.S., Aganova O.V., Yulmetov A.R., Filippov A.V., Antzutkin O.N., Gizatullin B.I., Afonin S., Klochkov V.V. J. Molecular Structure 1033, 59 (2013)
9. Usachev K.S., Efimov S.V., Yulmetov A.R., Filippov A.V., Antzutkin O.N., Afonin S., Klochkov V.V. Magn. Reson. Chem. 50(12), 784 (2012)
10. Usachev K.S., Filippov A.V., Antzutkin O.N., Klochkov V.V. Advances in Alzheimer’s Disease. 1(3), 22 (2012)
11. Klochkov V.V., Baikeev R.F., Skirda V.D., Klochkov A.V., Muhamadiev F.R., Baskyr I., Berger S. Magn. Reson. Chem. 47, 57 (2009)
12. Berger S., Braun S. 200 and More NMR Experiments, Wiley-VCH, Weinheim (2004)
13. Wuthrich K. NMR of Proteins and Nucleic Acids, Wiley-VCH, NY (1986)
14. Diaz M.D., Berger S. Magn. Reson. Chem. 39, 369 (2001)
15. Alba E., Tjandra N. Progress in NMR Spectroscopy 40, 175 (2002)
16. Gadiev T.A., Khairutdinov B.I., Antipin I.S., Klochkov V.V. Appl. Magn. Reson. 30(2), 65 (2006)
17. Schwieters C.D., Kuszewski J.J., Tjandra N., Clore G.M. J. Magn. Reson. 160, 65 (2003)
Review
For citations:
Blokhin D.S., Berger S., Klochkov V.V. Spatial structure of tetrapeptide N-AC-Ser-Phe-Val-Gly-OMe in "protein-micelle of sodium dodecyl sulfate" complex and in solid state by NMR spectroscopy. Magnetic Resonance in Solids. 2013;15(2):13202 (7 pp.).
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