Investigation of "cholesterol + model of biological membrane" complex by NMR spectroscopy
Abstract
On the basis of the nuclear magnetic resonance (NMR) experiments it was established that molecules of sodium dodecyl sulfate can form micelles in dimethyl sulfoxide solution. The nuclear Overhauser effect between OH-group of cholesterol and "tail" groups of sodium dodecyl sulfate hydrophobic part was observed in 1D selective NOESY experiment. This observation corresponds to close spatial arrangement of these parts of different molecules and the presence of a complex between cholesterol and sodium dodecyl sulfate micelles.
Keywords
nuclear magnetic resonance spectroscopy,
cholesterol,
sodium dodecyl sulfate,
micelles,
nuclear Overhauser effect
Supporting agencies: The work was supported by Federal Program of the Ministry of Education and Science of the Russian Federation (state contract no. 16.552.11.7083) and by Academy of Science of the Republic of Tatarstan (project no. 12-03-97040).
About the Authors
L. F. Galiullina
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya, 18, Kazan 420008
D. S. Blokhin
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya, 18, Kazan 420008
A. V. Aganov
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya, 18, Kazan 420008
V. V. Klochkov
Kazan Federal University
Russian Federation
Russian Federation
Kremlevskaya, 18, Kazan 420008
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Review
For citations:
Galiullina L.F., Blokhin D.S., Aganov A.V., Klochkov V.V. Investigation of "cholesterol + model of biological membrane" complex by NMR spectroscopy. Magnetic Resonance in Solids. 2012;14(2):12204 (7 pp.).
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